Exercise and supplementation with anti-obesity herbal extract on IL-6 and FNCD5 genes in the soleus muscle of obese female rats
DOI:
https://doi.org/10.47197/retos.v65.109550Keywords:
Aerobic exercise, fibron-ectin type iii domain containing, interleukin-6, obesity, resistance exerciseAbstract
Introduction and Objective: Exercise and anti-obesity herbs may be effective interventions to reduce high-fat diet (HFD)-induced obesity. Our study examined the effects of exercise (aerobic/AE and resistance/RE) on FNDC5 and IL-6 expression in HFD-obese female rats supplemented with anti-obesity herbal extract (AOHME).
Methodology: Forty-two young Wistar female rats (aged 12 weeks) were randomly distributed into seven groups: (1) Control rats with a standard diet (Con-ND), (2) Control rats with HFD (Con-HFD), (3) AE-HFD, (4) RE-HFD, (5) AOHME-HFD, (6) AE-AOHME-HFD, and (7) RE-AOHME-HFD. Rats were fed with HFD for four weeks (except Con-ND), followed by four weeks of HFD (groups 2-7). AE consisted of treadmill running and RE of stair climbing. Exercises were performed 5 times/week. Supplementation (100 mg AOHME/kg, 1h post-exercise) administered in gavage and distilled water as placebo only to HFD. Rats were euthanized (48h after the last intervention) and soleus muscle removed.
Results: HFD administration induced significant body weight increase compared to Con-ND group. Weight increase was noticed as well in HFD group compared to AE-HFD, RE-HFD, AOHME-HFD, AE-AOHME-HFD, and RE-AOHME-HFD. FNDC5 and IL-6 genes presented significant higher expression in Con-ND and HFD groups compared to Con-HFD.
Conclusions: FNDC5 and IL-6 gene expression were repressed by HFD and restored by AE and RE alone or in combination with AOHME.
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