Eight weeks of physical activity significantly increases BDNF levels in high school students in Indonesia: a randomized controlled trial

Authors

DOI:

https://doi.org/10.47197/retos.v73.117944

Keywords:

Physical Activity, Health, Physical Exercise, BDNF

Abstract

Background: Many factors influence cognitive function. One such factor is regular exercise. Previous studies have shown that acute physical activity increases BDNF, a biomarker of cognitive function. However, chronic exercise has not been widely discussed.

Objective: This study aims to determine the effect of eight weeks of physical activity on increasing BDNF levels in high school students in Indonesia.

Methods: This study involved 50 healthy women divided into 2 groups. The experimental group (CE) (n=25) and the control group (CO) (n=25) before and after being used in this experimental study. The study participants were between 17 and 20 years old. People were selected through random sampling, not forgetting they agreed to the informed consent given by the researcher to them as research respondents and after they were selected, two groups were formed, from which the treatment group (CE) did physical activity three times a week for eight weeks, and the control group (CO) did not receive any physical activity intervention. The study was conducted for eight weeks, starting with the collection of information about the characteristics of the subjects. The subjects were then instructed to do physical activities guided by professional physical education teachers. The training consisted of sports games including volleyball, basketball, and soccer. Before the training on the first day, blood was taken as pretest data, and after undergoing treatment for eight weeks, blood was taken again as posttest data.

Results: The results of the study showed that eight weeks of physical activity were proven to significantly increase BDNF levels in healthy women p < 0.05 *.

Conclusion: Therefore, it can be concluded that chronic physical activity can have a significant impact on cognitive function through BDNF levels. Therefore, regular physical exercise can be recommended to prevent cognitive decline.

References

Cho, H. C., Kim, J., Kim, S., Son, Y. H., Lee, N., & Jung, S. H. (2012). The concentrations of serum, plasma and platelet BDNF are all increased by treadmill VO 2max performance in healthy college men. Neuroscience Letters, 519(1), 78–83. https://doi.org/10.1016/j.neulet.2012.05.025

Cho, S. Y., So, W. Y., & Roh, H. T. (2017). The effects of taekwondo training on peripheral Neuroplasticity-Related growth factors, cerebral blood flow velocity, and cognitive functions in healthy children: A randomized controlled trial. International Journal of Environmental Research and Public Health, 14(5), 1–10. https://doi.org/10.3390/ijerph14050454

Dany, D., Arianti, D., Rossa, M., Aji Prayitno, D., Erfarenata, F., & Cahyanto Wibawa, J. (2025). Physiological responses of resistance training in increasing brain-derived neurotrophic factor levels: a systematic review. Retos, 68, 1250–1261. https://doi.org/10.47197/retos.v68.115912

Devenney, K. E., Guinan, E. M., Kelly, Á. M., Mota, B. C., Walsh, C., Olde Rikkert, M., Schneider, S., & Lawlor, B. (2019). Acute high-intensity aerobic exercise affects brain-derived neurotrophic factor in mild cognitive impairment: A randomised controlled study. BMJ Open Sport and Exercise Medicine, 5(1), 1–8. https://doi.org/10.1136/bmjsem-2018-000499

Ding, Q., Vaynman, S., Akhavan, M., Ying, Z., & Gomez-Pinilla, F. (2006). Insulin-like growth factor I interfaces with brain-derived neurotrophic factor-mediated synaptic plasticity to modulate aspects of exercise-induced cognitive function. Neuroscience, 140(3), 823–833. https://doi.org/10.1016/j.neuroscience.2006.02.084

El Hayek, L., Khalifeh, M., Zibara, V., Abi Assaad, R., Emmanuel, N., Karnib, N., El-Ghandour, R., Nasrallah, P., Bilen, M., Ibrahim, P., Younes, J., Abou Haidar, E., Barmo, N., Jabre, V., Stephan, J. S., & Sleiman, S. F. (2019). 17. El lactato media los efectos del ejercicio sobre el aprendizaje y la memoria através de la activación dependiente de SIRT1 del factor neurotrófico derivado delcerebro (BDNF) del hipocampo. — Lactate mediates the effects of exercise on learning and. Journal of Neuroscience, 39(13), 2369–2382.

Erickson, K. I., Miller, D. L., & Roecklein, K. A. (2012). The aging hippocampus: Interactions between exercise, depression, and BDNF. Neuroscientist, 18(1), 82–97. https://doi.org/10.1177/1073858410397054

Erickson, K. I., Voss, M. W., Prakash, R. S., Basak, C., Szabo, A., Chaddock, L., Kim, J. S., Heo, S., Alves, H., White, S. M., Wojcicki, T. R., Mailey, E., Vieira, V. J., Martin, S. A., Pence, B. D., Woods, J. A., McAuley, E., & Kramer, A. F. (2011). Exercise training increases size of hippocampus and improves memory. Proceedings of the National Academy of Sciences of the United States of America, 108(7), 3017–3022. https://doi.org/10.1073/pnas.1015950108

Farmer, J., Zhao, X., Van Praag, H., Wodtke, K., Gage, F. H., & Christie, B. R. (2004). Effects of voluntary exercise on synaptic plasticity and gene expression in the dentate gyrus of adult male sprague-dawley rats in vivo. Neuroscience, 124(1), 71–79. https://doi.org/10.1016/j.neuroscience.2003.09.029

Gale, S. A., Acar, D., & Daffner, K. R. (2018). Dementia. American Journal of Medicine, 131(10), 1161–1169. https://doi.org/10.1016/j.amjmed.2018.01.022

Gogniat, M. A., Robinson, T. L., Jean, K. R., & Stephen Miller, L. (2022). Physical activity moderates the association between executive function and functional connectivity in older adults. Aging Brain, 2, 100036. https://doi.org/10.1016/j.nbas.2022.100036

Griffin, É. W., Mullally, S., Foley, C., Warmington, S. A., O’Mara, S. M., & Kelly, Á. M. (2011). Aerobic exercise improves hippocampal function and increases BDNF in the serum of young adult males. Physiology and Behavior, 104(5), 934–941. https://doi.org/10.1016/j.physbeh.2011.06.005

Haapasalo, A., Sipola, I., Larsson, K., Åkerman, K. E. O., Stoilov, P., Stamm, S., Wong, G., & Castrén, E. (2002). Regulation of TRKB surface expression by brain-derived neurotrophic factor and truncated TRKB isoforms. Journal of Biological Chemistry, 277(45), 43160–43167. https://doi.org/10.1074/jbc.M205202200

Hötting, K., Schickert, N., Kaiser, J., Röder, B., & Schmidt-Kassow, M. (2016). The effects of acute physical exercise on memory, peripheral BDNF, and cortisol in young adults. Neural Plasticity, 2016. https://doi.org/10.1155/2016/6860573

Iso-Markku, P., Kujala, U. M., Knittle, K., Polet, J., Vuoksimaa, E., & Waller, K. (2022). Physical activity as a protective factor for dementia and Alzheimer’s disease: systematic review, meta-analysis and quality assessment of cohort and case-control studies. British Journal of Sports Medicine, 56(12), 701–709. https://doi.org/10.1136/bjsports-2021-104981

Jeon, Y. K., & Ha, C. H. (2017). The effect of exercise intensity on brain derived neurotrophic factor and memory in adolescents. Environmental Health and Preventive Medicine, 22(1), 1–6. https://doi.org/10.1186/s12199-017-0643-6

Jiang, N., Lv, J., Zhang, Y., Sun, X., Yao, C., Wang, Q., He, Q., & Liu, X. (2023). Protective effects of ginsenosides Rg1 and Rb1 against cognitive impairment induced by simulated microgravity in rats. Frontiers in Pharmacology, 14(April), 1–11. https://doi.org/10.3389/fphar.2023.1167398

Lammers, M. D., Aneli, N. M., de Oliveira, G. G., de Oliveira Maciel, S. F. V., Zanini, D., Manica, A., de Resende e Silva, D. T., Bagatini, M. D., Sevigny, J., De Sa, C. A., Manfredi, L. H., & Cardoso, A. M. (2020). The anti-inflammatory effect of resistance training in hypertensive women: The role of purinergic signaling. Journal of Hypertension, 38(12), 2490–2500. https://doi.org/10.1097/HJH.0000000000002578

Lima Giacobbo, B., Doorduin, J., Klein, H. C., Dierckx, R. A. J. O., Bromberg, E., & de Vries, E. F. J. (2019). Brain-Derived Neurotrophic Factor in Brain Disorders: Focus on Neuroinflammation. Molecular Neurobiology, 56(5), 3295–3312. https://doi.org/10.1007/s12035-018-1283-6

Lippi, G., Mattiuzzi, C., & Sanchis-Gomar, F. (2020). Updated overview on interplay between physical exercise, neurotrophins, and cognitive function in humans. Journal of Sport and Health Science, 9(1), 74–81. https://doi.org/10.1016/j.jshs.2019.07.012

Long, S., Benoist, C., & Weidner, W. (2023). World Alzheimer Report 2023. 94.

Lukkahatai, N., Ong, I. L., Benjasirisan, C., & Saligan, L. N. (2025). Brain-Derived Neurotrophic Factor (BDNF) as a Marker of Physical Exercise or Activity Effectiveness in Fatigue, Pain, Depression, and Sleep Disturbances: A Scoping Review. Biomedicines, 13(2), 1–21. https://doi.org/10.3390/biomedicines13020332

Miranda, M., Morici, J. F., Zanoni, M. B., & Bekinschtein, P. (2019). Brain-Derived Neurotrophic Factor: A Key Molecule for Memory in the Healthy and the Pathological Brain. Frontiers in Cellular Neuroscience, 13(August), 1–25. https://doi.org/10.3389/fncel.2019.00363

Nichols, E., Steinmetz, J. D., Vollset, S. E., Fukutaki, K., Chalek, J., Abd-Allah, F., Abdoli, A., Abualhasan, A., Abu-Gharbieh, E., Akram, T. T., Al Hamad, H., Alahdab, F., Alanezi, F. M., Alipour, V., Almustanyir, S., Amu, H., Ansari, I., Arabloo, J., Ashraf, T., … Vos, T. (2022). Estimation of the global prevalence of dementia in 2019 and forecasted prevalence in 2050: an analysis for the Global Burden of Disease Study 2019. The Lancet Public Health, 7(2), e105–e125. https://doi.org/10.1016/S2468-2667(21)00249-8

Nichols, E., Szoeke, C. E. I., Vollset, S. E., Abbasi, N., Abd-Allah, F., Abdela, J., Aichour, M. T. E., Akinyemi, R. O., Alahdab, F., Asgedom, S. W., Awasthi, A., Barker-Collo, S. L., Baune, B. T., Béjot, Y., Belachew, A. B., Bennett, D. A., Biadgo, B., Bijani, A., Bin Sayeed, M. S., … Murray, C. J. L. (2019). Global, regional, and national burden of Alzheimer’s disease and other dementias, 1990–2016: a systematic analysis for the Global Burden of Disease Study 2016. The Lancet Neurology, 18(1), 88–106. https://doi.org/10.1016/S1474-4422(18)30403-4

Pickett, J., & Brayne, C. (2019). The scale and profile of global dementia research funding. The Lancet, 394(10212), 1888–1889. https://doi.org/10.1016/S0140-6736(19)32599-1

Piepmeier, A. T., Etnier, J. L., Wideman, L., Berry, N. T., Kincaid, Z., & Weaver, M. A. (2020). A preliminary investigation of acute exercise intensity on memory and BDNF isoform concentrations. European Journal of Sport Science, 20(6), 819–830. https://doi.org/10.1080/17461391.2019.1660726

Pollán, M., Casla-Barrio, S., Alfaro, J., Esteban, C., Segui-Palmer, M. A., Lucia, A., & Martín, M. (2020). Exercise and cancer: a position statement from the Spanish Society of Medical Oncology. Clinical and Translational Oncology, 22(10), 1710–1729. https://doi.org/10.1007/s12094-020-02312-y

Putra, D. P., Wibawa, J. C., & Putro, B. N. (2025). Physical exercise as a key to activating fat burning through the activation of uncoupling protein 1 (ucp1) in adipose tissue: a scoping review. Retos, 67, 1061–1075. https://doi.org/10.47197/retos.v67.111997

Reycraft, J. T., Islam, H., Townsend, L. K., Hayward, G. C., Hazell, T. O. M. J., & MacPherson, R. E. K. (2020). Exercise Intensity and Recovery on Circulating Brain-derived Neurotrophic Factor. Medicine and Science in Sports and Exercise, 52(5), 1210–1217. https://doi.org/10.1249/MSS.0000000000002242

Ribeiro, D., Petrigna, L., Pereira, F. C., Muscella, A., Bianco, A., & Tavares, P. (2021). The impact of physical exercise on the circulating levels of BDNF and NT 4/5: A review. International Journal of Molecular Sciences, 22(16). https://doi.org/10.3390/ijms22168814

Roig, M., Nordbrandt, S., Geertsen, S. S., & Nielsen, J. B. (2013). The effects of cardiovascular exercise on human memory: A review with meta-analysis. Neuroscience and Biobehavioral Reviews, 37(8), 1645–1666. https://doi.org/10.1016/j.neubiorev.2013.06.012

Romero Garavito, A., Díaz Martínez, V., Juárez Cortés, E., Negrete Díaz, J. V., & Montilla Rodríguez, L. M. (2024). Impact of physical exercise on the regulation of brain-derived neurotrophic factor in people with neurodegenerative diseases. Frontiers in Neurology, 15(January), 1–16. https://doi.org/10.3389/fneur.2024.1505879

Sáenz Jiménez, C. (2021). Beneficios del Ejercicio Físico sobre la Neuroplasticidad y la Cognición. NeuroRehabNews, 2(Octubre), 1–2. https://doi.org/10.37382/nrn.octubre.2017.524

Sleiman, S. F., & Chao, M. V. (2015). Downstream Consequences of Exercise Through the Action of BDNF. Brain Plasticity, 1(1), 143–148. https://doi.org/10.3233/bpl-150017

Tsai, S. J. (2018). Critical issues in BDNF Val66met genetic studies of neuropsychiatric disorders. Frontiers in Molecular Neuroscience, 11(May), 1–15. https://doi.org/10.3389/fnmol.2018.00156

Tyler, W. J., & Pozzo-Miller, L. D. (2001). BDNF Enhances Quantal Neurotransmitter Release and Increases the Number of Docked Vesicles at the Active Zones of Hippocampal Excitatory Synapses, The journal of Neuroscience, 21, 4249-4258, doi: 10.1523/JNEUROSCI.21-12-04249.2001. 21(12), 4249–4258.

Ventriglia, M., Zanardini, R., Bonomini, C., Zanetti, O., Volpe, D., Pasqualetti, P., Gennarelli, M., & Bocchio-Chiavetto, L. (2013). Serum brain-derived neurotrophic factor levels in different neurological diseases. BioMed Research International, 2013. https://doi.org/10.1155/2013/901082

Vivar, C., Peterson, B. D., & van Praag, H. (2016). Running rewires the neuronal network of adult-born dentate granule cells. NeuroImage, 131, 29–41. https://doi.org/10.1016/j.neuroimage.2015.11.031

Wan, J., Ma, L., Jiao, X., Dong, W., Lin, J., Qiu, Y., Wu, W., Liu, Q., Chen, C., Huang, H., Li, S., Zheng, H., & Wu, Y. (2024). Impaired synaptic plasticity and decreased excitability of hippocampal glutamatergic neurons mediated by BDNF downregulation contribute to cognitive dysfunction in mice induced by repeated neonatal exposure to ketamine. CNS Neuroscience and Therapeutics, 30(2), 1–18. https://doi.org/10.1111/cns.14604

Wang, W. H., He, G. P., Xiao, X. P., Gu, C., & Chen, H. Y. (2012). Relationship between brain-derived neurotrophic factor and cognitive function of obstructive sleep apnea/hypopnea syndrome patients. Asian Pacific Journal of Tropical Medicine, 5(11), 906–910. https://doi.org/10.1016/S1995-7645(12)60169-2

Weaver, S. R., Skinner, B. D., Furlong, R., Lucas, R. A. I., Cable, N. T., Rendeiro, C., McGettrick, H. M., & Lucas, S. J. E. (2021). Cerebral Hemodynamic and Neurotrophic Factor Responses Are Dependent on the Type of Exercise. Frontiers in Physiology, 11(January), 1–14. https://doi.org/10.3389/fphys.2020.609935

Won, J., Callow, D. D., Pena, G. S., Gogniat, M. A., Kommula, Y., Arnold-Nedimala, N. A., Jordan, L. S., & Smith, J. C. (2021). Evidence for exercise-related plasticity in functional and structural neural network connectivity. Neuroscience and Biobehavioral Reviews, 131(301), 923–940. https://doi.org/10.1016/j.neubiorev.2021.10.013

Xu, L., Zhu, L., Zhu, L., Chen, D., Cai, K., Liu, Z., & Chen, A. (2021). Moderate exercise combined with enriched environment enhances learning and memory through bdnf/trkb signaling pathway in rats. International Journal of Environmental Research and Public Health, 18(16). https://doi.org/10.3390/ijerph18168283

Zhao, T., Pan, P., Zhou, Y., Zhang, X., Li, Q., & Zhou, Y. (2025). Age-sex differences in Alzheimer’s and related dementias burden and risk factors in east and Southeast Asia: results from the 2021 GBD study. Frontiers in Aging Neuroscience, 17(June), 1–12. https://doi.org/10.3389/fnagi.2025.1562148

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11-11-2025

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Soleh Fudin, M., Febrianto, N., Budi Santosa, A., & Cahyanto Wibawa, J. (2025). Eight weeks of physical activity significantly increases BDNF levels in high school students in Indonesia: a randomized controlled trial. Retos, 73, 1368-1378. https://doi.org/10.47197/retos.v73.117944