Cold-water immersion for reducing IL-6 and CK in female footballers
DOI:
https://doi.org/10.47197/retos.v70.110245Keywords:
Cold Water Immersion, creatine kinase, interleukin-6, vertical jump, female soccer playerAbstract
Introduction: Soccer requires athletes to possess strength and endurance, involving intense physical activities that may lead to fatigue and injury. Such conditions stimulate the release of inflammatory markers, including interleukin-6 (IL-6) and creatine kinase (CK), which can impair recovery and athletic performance. Cold-water immersion (CWI) is a commonly used recovery strategy to mitigate these effects.
Objective: This study aimed to examine the impact of CWI on IL-6 and CK levels, as well as vertical jump performance in female soccer players following a match.
Methodology: A quasi-experimental two-group pretest-posttest design was employed, involving 20 purposively sampled participants. Blood samples were collected at four time points: pre-match, post-match, 24 hours post-match, and 48 hours post-match. Vertical jump performance was used to assess physical recovery.
Results: Results showed no significant difference in vertical jump performance between the groups at 24 hours post-match (p > 0.05), but a significant difference was found at 48 hours post-match (p < 0.05), favoring the CWI group. Significant differences in IL-6 and CK levels were observed between the groups at both 24 and 48 hours post-match (p < 0.05).
Discussion: CWI effectively reduced inflammation and muscle damage, as reflected in IL-6 and CK values, and promoted faster neuromuscular recovery. This finding suggests that CWI supports biological repair and functional recovery within 48 hours post-match.
Conclusions: Cold-water immersion significantly improved physiological recovery in female soccer players, as indicated by lower IL-6 and CK levels and improved vertical jump performance over time compared to the control group.
References
Aguiar, P. F., Magalhães, S. M., Fonseca, I. A. T., da Costa Santos, V. B., de Matos, M. A., Peixoto, M. F. D., Nakamura, F. Y., Crandall, C., Araújo, H. N., Silveira, L. R., Rocha-Vieira, E., de Castro Magalhães, F., & Amorim, F. T. (2016). Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers. Cell Stress & Chaperones, 21(5), 793–804. https://doi.org/10.1007/s12192-016-0704-6
Almeida, P. S. M. de, Scotta, Â. P., Pimentel, B. de M., Júnior, S. B., & Sampaio, Y. R. (2013). Incidência de lesão musculoesquelética em jogadores de futebol. Revista Brasileira de Medicina Do Es-porte, 19(2), 112–115. https://doi.org/10.1590/s1517-86922013000200008
Ascensão, A., Leite, M., Rebelo, A. N., Magalhäes, S., & Magalhäes, J. (2011). Effects of cold water im-mersion on the recovery of physical performance and muscle damage following a one-off soc-cer match. Journal of Sports Sciences, 29(3), 217–225. https://doi.org/10.1080/02640414.2010.526132
Binat, M., & Kartiko, D. (2021). Minat Siswi SMA Kemala Bayangkari 1 Surabaya Dalam Permainan Sepak Bola. Jurnal Pendidikan Olahraga Dan Kesehatan, 09(03), 19–24. https://ejournal.unesa.ac.id/index.php/jurnal-pendidikan-jasmani/issue/archivehttps://ejournal.unesa.ac.id/index.php/jurnal-pendidikan-jasmani
BOSCO, C., KOMI, P. V., & ITO, A. (1981). Prestretch potentiation of human skeletal muscle during bal-listic movement. Acta Physiologica Scandinavica, 111(2), 135–140. https://doi.org/10.1111/j.1748-1716.1981.tb06716.x
Cormie, P., Mccaulley, G. O., & Mcbride, J. M. (2007). Power Versus Strength-Power Jump Squat Train-ing. Medicine & Science in Sports & Exercise, 39(6), 996–1003. https://doi.org/10.1097/mss.0b013e3180408e0c
Dannecker, E. A., Liu, Y., Rector, R. S., Thomas, T. R., Fillingim, R. B., & Robinson, M. E. (2012). Sex Dif-ferences in Exercise-Induced Muscle Pain and Muscle Damage. The Journal of Pain, 13(12), 1242–1249. https://doi.org/10.1016/j.jpain.2012.09.014
Darani, M. M., Abedi, B., & Fatolahi, H. (2018). The effect of active and passive recovery on creatine kinase and C-reactive protein after an exercise session in football players. International Ar-chives of Health Sciences, 5(1), 1. https://doi.org/10.4103/iahs.iahs_31_17
Dewangga, M. W., Irianto, D. P., Dimyati, Sumaryanto, Nasihun, T., Febrianta, Y., Wahyuni, Wijianto, & Agustiyawan. (2021). Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats. Journal of Kerman University of Medical Sciences, 28(6), 539–547. https://doi.org/10.22062/jkmu.2021.91825
Dewangga, M. W., Susilo, T. E., Cahyadi, M. M., Saputra, H., Furqony, I. Y., Putra, Z. Y., Wilger, R. V., Candrika, A. A., & Rosyid, F. N. (2024). Physical performance and markers of muscle damage response after single soccer matches in amateur women’s soccer players. Fizjoterapia Polska, 24(2), 136–143. https://doi.org/10.56984/8ZG5608X8S
Doewes, R. I., & Nuryadin, I. (2022). Analysis of the participation of women in football in Indonesia. Sport TK, 11, 1–13. https://doi.org/10.6018/sportk.522901
Ebben, W. P., & Petushek, E. J. (2010). Using the Reactive Strength Index Modified to Evaluate Plyome-tric Performance. Journal of Strength and Conditioning Research, 24(8), 1983–1987. https://doi.org/10.1519/JSC.0b013e3181e72466
Hara, M., Shibayama, A., Takeshita, D., & Fukashiro, S. (2006). The effect of arm swing on lower ex-tremities in vertical jumping. Journal of Biomechanics, 39(13), 2503–2511. https://doi.org/10.1016/j.jbiomech.2005.07.030
Ihsan, M., Watson, G., & Abbiss, C. R. (2016). What are the Physiological Mechanisms for Post-Exercise Cold Water Immersion in the Recovery from Prolonged Endurance and Intermittent Exercise? Sports Medicine, 46(8), 1095–1109. https://doi.org/10.1007/s40279-016-0483-3
Lees, A., Vanrenterghem, J., & Clercq, D. De. (2004). Understanding how an arm swing enhances per-formance in the vertical jump. Journal of Biomechanics, 37(12), 1929–1940. https://doi.org/10.1016/j.jbiomech.2004.02.021
Lim, E., Miyamura, J., & Chen, J. J. (2015). Racial/Ethnic-Specific Reference Intervals for Common La-boratory Tests: A Comparison among Asians, Blacks, Hispanics, and White. Hawai’i Journal of Medicine & Public Health : A Journal of Asia Pacific Medicine & Public Health, 74(9), 302–310.
Machado, A. F., Ferreira, P. H., Micheletti, J. K., de Almeida, A. C., Lemes, Í. R., Vanderlei, F. M., Netto Junior, J., & Pastre, C. M. (2016). Can Water Temperature and Immersion Time Influence the Ef-fect of Cold Water Immersion on Muscle Soreness? A Systematic Review and Meta-Analysis. Sports Medicine, 46(4), 503–514. https://doi.org/10.1007/s40279-015-0431-7
Marangoni, L. S., Pottratz, S., & Boiangin, N. (2023). The Impacts of Burnout on Athletic Identity and Attitude towards Sport. Youth, 3(4), 1121–1136. https://doi.org/10.3390/youth3040071
Markovic, G., Dizdar, D., Jukic, I., & Cardinale, M. (2004). Reliability and Factorial Validity of Squat and Countermovement Jump Tests. The Journal of Strength and Conditioning Research, 18(3), 551. https://doi.org/10.1519/1533-4287(2004)18<551:RAFVOS>2.0.CO;2
Moir, G., Button, C., Glaister, M., & Stone, M. H. (2004). Influence of Familiarization on the Reliability of Vertical Jump and Acceleration Sprinting Performance in Physically Active Men. The Journal of Strength and Conditioning Research, 18(2), 276. https://doi.org/10.1519/R-13093.1
Montgomery, P. G., Pyne, D. B., Hopkins, W. G., Dorman, J. C., Cook, K. K., & Minahan, C. L. (2008). The effect of recovery strategies on physical performance and cumulative fatigue in competitive basketball. Journal of Sports Sciences, 26(11), 1135–1145. https://doi.org/10.1080/02640410802104912
Moore, E., Fuller, J. T., Buckley, J. D., Saunders, S., Halson, S. L., Broatch, J. R., & Bellenger, C. R. (2022). Impact of Cold-Water Immersion Compared with Passive Recovery Following a Single Bout of Strenuous Exercise on Athletic Performance in Physically Active Participants: A Systematic Re-view with Meta-analysis and Meta-regression. Sports Medicine, 52(7), 1667–1688. https://doi.org/10.1007/s40279-022-01644-9
Orunbayev, A. (2023). Recovery Strategy In Sports. American Journal Of Social Sciences And Humanity Research, 03(12), 135–147. https://doi.org/10.37547/ajsshr/Volume03Issue12-18
Ramos, Z. N. A., Colmenero, B. R. R., Dávila, M. Z. G., Maldonado, G. E. M., Flores, J. R. H., & Cruz, G. H. (2021). Influencia del masaje ZNAR y la inmersión en agua fría en el proceso inflamatorio, Cre-atin Kinasa y percepción al dolor muscular en jugadores de voleibol (Influence of ZNAR mas-sage and cold water immersion on the inflammatory process, Creatine Kinase and. Retos, 44, 95–102. https://doi.org/10.47197/retos.v44i0.89399
Rosell, D. R., Custodio, R. M., Márquez, F. F., García, J. M. Y., & Badillo, J. J. G. (2017). Traditional vs. Sport-Specific Vertical Jump Tests: Reliability, Validity, and Relationship With the Legs Strength and Sprint Performance in Adult and Teen Soccer and Basketball Players. Journal of Strength and Conditioning Research, 31(1), 196–206. https://doi.org/10.1519/JSC.0000000000001476
Sedano, S., & Maroto-Izquierdo, S. (2025). Effectiveness of Different Neuromuscular Recovery Strate-gies in Elite Youth Female Football Players. Sports, 13(2), 36. https://doi.org/10.3390/sports13020036
Tanaka, T., Narazaki, M., & Kishimoto, T. (2014). IL-6 in Inflammation, Immunity, and Disease. Cold Spring Harbor Perspectives in Biology, 6(10), a016295–a016295. https://doi.org/10.1101/cshperspect.a016295
Taneja, V. (2018). Sex Hormones Determine Immune Response. Frontiers in Immunology, 9(August), 1–5. https://doi.org/10.3389/fimmu.2018.01931
Teoldo, I., Dambroz, F., & Brito, J. (2024). Performance Of Soccer Players Under Acute Physical Fa-tigue: An Approach Based On Cognitive, Tactical And Physical Aspects. Heliyon, 10(9), e30516. https://doi.org/10.1016/j.heliyon.2024.e30516
Versey, N. G., Halson, S. L., & Dawson, B. T. (2013). Water immersion recovery for athletes: Effect on exercise performance and practical recommendations. Sports Medicine, 43(11), 1101–1130. https://doi.org/10.1007/s40279-013-0063-8
Wang, Z. R., & Ni, G. X. (2021). Is it Time to put Traditional Cold Therapy in Rehabilitation of Soft-tissue Injuries out to Pasture? World Journal of Clinical Cases, 9(17), 4116–4122. https://doi.org/10.12998/wjcc.v9.i17.4116
White, G. E., Rhind, S. G., & Wells, G. D. (2014). The effect of various cold-water immersion protocols on exercise-induced inflammatory response and functional recovery from high-intensity sprint exercise. European Journal of Applied Physiology, 114(11), 2353–2367. https://doi.org/10.1007/s00421-014-2954-2
Zhang, H., & Dhalla, N. S. (2024). The Role of Pro-Inflammatory Cytokines in the Pathogenesis of Car-diovascular Disease. International Journal of Molecular Sciences, 25(2). https://doi.org/10.3390/ijms25021082
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Mahendra Wahyu Dewangga, Anindita Antya Candrika, Tri Novaliano Rechtsi Medistianto, Ribka Vlorentyna Wilger

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and ensure the magazine the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of authorship of the work and the initial publication in this magazine.
- Authors can establish separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Is allowed and authors are encouraged to disseminate their work electronically (eg, in institutional repositories or on their own website) prior to and during the submission process, as it can lead to productive exchanges, as well as to a subpoena more Early and more of published work (See The Effect of Open Access) (in English).
This journal provides immediate open access to its content (BOAI, http://legacy.earlham.edu/~peters/fos/boaifaq.htm#openaccess) on the principle that making research freely available to the public supports a greater global exchange of knowledge. The authors may download the papers from the journal website, or will be provided with the PDF version of the article via e-mail.