High intensity physical activities increase troponin I level but does not affect changing in glutathione peroxidase in mice
DOI:
https://doi.org/10.47197/retos.v65.110561Keywords:
alta intensidad, intensidad moderada, glutatión peroxidasa, troponina IAbstract
Introduction: Muscle damage can be triggered by other free radicals formed during intense physical activites and the low response of antioxidants. The antioxidants that plays a role in responding to free radicals during physical activites is glutathione peroxidase.
Objective: The main purpose of this study was to analyze the effects of high-intensity and moderate-intensity physical activities on muscle glutathione peroxidase (GPx) and serum troponin I levels as markers of damage.
Methodology: The study was used a quantitative true experiment with a post-test only group design. This study was conducted at a total of 18 healthy mice with age of 8 weeks old male Balb/c mice, then divided into 3 groups: high-intensity physical activity (HI), moderate-intensity physical activity (MI), and control (CON) groups. The HI group performed running on a treadmill with a 90% of their maximum speed, while the MI group at 60%. Data was taken of 24 hours post-physical activity by taking blood samples to examine troponin I levels and skeletal muscle gastrocnemius homogenate to determine GPx activity.
Results: the study found that the MI group had a higher concentration of GPx in the skeletal muscle gastrocnemius compared to the other control group. However, the concentration of troponin I showed significant differences between the groups, HI group had significantly higher levels of troponin I compared to the other groups.
Conclusion: The high-intensity physical activity has the highest result in ncreasing Troponin I and the moderate intensity physical activity has the highest concentration of glutathione peroxidase compare to other groups.
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