Modulation of exercise-induced oxidative stress biomarkers  by nutraceutical moringa oleifera supplementation in football athletes: a randomized double-blind trial

Authors

DOI:

https://doi.org/10.47197/retos.v74.117332

Keywords:

resistance training, antioxidants response, oxidative damage, exercise recovery, nutritional strategy

Abstract

Introduction: Redox homeostasis is disrupted by the increased intensity of physical exercise, leading to elevated levels of reactive oxygen species (ROS). This oxidative imbalance is characterized by reduced antioxidant enzyme activity, such as superoxide dismutase (SOD), and increased lipid peroxidation markers like malondialdehyde (MDA). Moringa oleifera (MO), rich in polyphenols and flavonoids, is believed to exert antioxidant effects and alleviate oxidative stress.

Objective: This study aimed to assess the acute effects of MO supplementation on oxidative stress biomarkers following maximal load exercise in trained football athletes.

Method: A randomized, double-blind, placebo-controlled trial was conducted involving 22 trained male football athletes. Participants received either 1000 mg of MO or a placebo before performing high-intensity squat thrusts to exhaustion. Data were analyzed using paired t-tests and Pearson correlation (p < 0.05).

Results: Baseline data showed no significant differences, but the MO group significantly elevated SOD and lower MDA levels compared to the placebo at 1 and 24 hours post-exercise  (p < 0.01), with strong negative correlations between SOD and MDA 1 hour (r = –0.50, p = 0.02) and 24 hours (r = –0.71, p < 0.001).

Discussion: Acute MO supplementation enhances endogenous antioxidant defense and reduces oxidative damage following maximal exertion.

Conclusion: These findings support the use of MO as a locally sourced recovery aid in sports, particularly in mitigating oxidative stress in high-performance athletes.

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11-12-2025

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Mustar, Y. S., Pramono, B. A., Muhammad, M., Kuo, C.-H., Kusnanik, N. W., & Hariyanto, A. (2025). Modulation of exercise-induced oxidative stress biomarkers  by nutraceutical moringa oleifera supplementation in football athletes: a randomized double-blind trial. Retos, 74, 810-821. https://doi.org/10.47197/retos.v74.117332