Pilot study on the effectiveness of Sidhakarya meditation in reducing malondialdehyde levels: an experimental approach
DOI:
https://doi.org/10.47197/retos.v66.111837Keywords:
Oxidative stress, Malondialdehyde, Sidhakarya MeditationAbstract
Introduction: Oxidative stress, caused by an imbalance between reactive oxygen species (ROS) and antioxidants, contributes to chronic diseases. Malondialdehyde (MDA), a marker of oxidative damage, is commonly used to assess this stress. Meditation, especially mindfulness practices, has shown potential in reducing oxidative stress. This pilot study investigates the impact of Sidhakarya Meditation, a specific mindfulness-based technique, on MDA levels in individuals experiencing moderate stress.
Objective: To evaluate the effectiveness of Sidhakarya Meditation in reducing MDA levels, a key biomarker of oxidative stress.
Methodology: A randomized pre-test and post-test control group design was employed. A total of 60 participants (mean age 34.5 ± 8.2 years) were randomly assigned to the intervention group (Group B), which practiced daily 30-minute Sidhakarya meditation sessions for 6 weeks, or the control group (Group A), which maintained their usual activities. MDA levels were measured using the thiobarbituric acid reactive substances (TBARS) assay. Psychological stress and mood were assessed using the Perceived Stress Scale (PSS) and the Profile of Mood States (POMS).
Results: Of the 60 participants, 55 completed the study. The intervention group showed a 30% reduction in MDA levels (from 1780 nmol/mL to 1242 nmol/mL, p = 0.01), along with significant improvements in stress (p = 0.04) and mood (p = 0.03).
Discussion: Sidhakarya Meditation effectively reduced oxidative stress and improved emotional well-being, supporting its potential as a non-pharmacological intervention for stress management.
Conclusions: This study highlights Sidhakarya Meditation as a promising approach for reducing oxidative stress and improving psychological health.
References
Büssing, A., Michalsen, A., Khalsa, S. B. S., Telles, S., & Sherman, K. J. (2012). Effects of yoga on mental and physical health: A short summary of reviews. In Evidence-based Complementary and Alternative Medicine (Vol. 2012). https://doi.org/10.1155/2012/165410
Chmielewski, J., Łoś, K., & Łuczyński, W. (2021). Mindfulness in healthcare professionals and medical education. In International Journal of Occupational Medicine and Environmental Health (Vol. 34, Issue 1, pp. 1–14). Nofer Institute of Occupational Medicine. https://doi.org/10.13075/ijomeh.1896.01542
Chen, J., Zeng, L., Xia, T., Li, S., Yan, T., Wu, S., Qiu, G., & Liu, Z. (2015). Toward a biomarker of oxidative stress: a fluorescent probe for exogenous and endogenous malondialdehyde in living cells. Analytical chemistry, 87(16), 8052–8056. https://doi.org/10.1021/acs.analchem.5b02032
Cohen, S., Gianaros, P. J., & Manuck, S. B. (2016). A Stage Model of Stress and Disease. Perspectives on Psychological Science, 11(4), 456–463. https://doi.org/10.1177/1745691616646305
Dimitrios Tsikas. (2017). Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Analytical Biochemistry, 524, 13–30. https://doi.org/10.1016/j.ab.2016.10.021
Goyal, M., Singh, S., Sibinga, E. M. S., Gould, N. F., Rowland-Seymour, A., Sharma, R., Berger, Z., Sleicher, D., Maron, D. D., Shihab, H. M., Ranasinghe, P. D., Linn, S., Saha, S., Bass, E. B., & Haythornthwaite, J. A. (2014). Meditation programs for psychological stress and well-being: A systematic review and meta-analysis. JAMA Internal Medicine, 174(3), 357–368. https://doi.org/10.1001/jamainternmed.2013.13018
Househam, A. M., Peterson, C. T., Mills, P. J., & Chopra, D. (2017). The Effects of Stress and Meditation on the Immune System, Human Microbiota, and Epigenetics. Advances in Mind-Body Medicine, 31(4), 10–25.
Incalza, M. A., D’Oria, R., Natalicchio, A., Perrini, S., Laviola, L., & Giorgino, F. (2018). Oxidative stress and reactive oxygen species in endothelial dysfunction associated with cardiovascular and metabolic diseases. In Vascular Pharmacology (Vol. 100, pp. 1–19). Elsevier Inc. https://doi.org/10.1016/j.vph.2017.05.005
Indra Wirawan, K. (2021). Theo-Esthetic-Philosophy of Topeng Sidakarya in Balinese Hindu Religious Practice. Mudra Jurnal Seni Budaya, 36(2), 230–236. https://doi.org/doi:10.31091/mudra.v36i2.1283.
Khoury, B., Sharma, M., Rush, S. E., & Fournier, C. (2015). Mindfulness-based stress reduction for healthy individuals: A meta-analysis. Journal of Psychosomatic Research, 78(6), 519–528. https://doi.org/https://doi.org/10.1016/j.jpsychores.2015.03.009
Kim, D. H., Moon, Y. S., Kim, H. S., Jung, J. S., Park, H. M., Suh, H. W., Kim, Y. H., & Song, D. K. (2005). Effect of Zen Meditation on serum nitric oxide activity and lipid peroxidation. Progress in neuro-psychopharmacology & biological psychiatry, 29(2), 327–331. https://doi.org/10.1016/j.pnpbp.2004.11.019
Marshall, M., McClanahan, M., Warren, S. M., Rogers, R., & Ballmann, C. (2020). A comparison of the acute effects of different forms of yoga on physiological and psychological stress: A pilot study. International Journal of Environmental Research and Public Health, 17(17), 1–8. https://doi.org/10.3390/ijerph17176090
Mas-Bargues, C., Escrivá, C., Dromant, M., Borrás, C., & Viña, J. (2021). Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease. In Archives of Biochemistry and Biophysics (Vol. 709). Academic Press Inc. https://doi.org/10.1016/j.abb.2021.108941
Oka, T., & Lkhagvasuren, B. (2021). Health-related benefits and adverse events associated with yoga classes among participants that are healthy, in poor health, or with chronic diseases. BioPsychoSocial Medicine, 15(1). https://doi.org/10.1186/s13030-021-00216-z
Oken, B. S., Goodrich, E., Klee, D., Research Assistant, B. [, Memmott, T., & Proulx, J. (2018). Predictors of Improvements in Mental Health From Mindfulness Meditation in Stressed Older Adults. In Altern Ther Health Med (Vol. 24, Issue 1).
Patil, Y., Sabbu, K., Iyer, R. B., Philip, S. T., Armila Nadhar, A., Thakur, K. S., Kadu, P., & Thakur, M. (2024). Effect of Heartfulness Meditation on Oxidative Stress and Mindfulness in Healthy Participants. Cureus, 16(6), e62943. https://doi.org/10.7759/cureus.62943
Pascoe, M. C., Thompson, D. R., & Ski, C. F. (2017). Yoga, mindfulness-based stress reduction and stress-related physiological measures: A meta-analysis. In Psychoneuroendocrinology (Vol. 86, pp. 152–168). Elsevier Ltd. https://doi.org/10.1016/j.psyneuen.2017.08.008
Santosa, I. G. N. P. E., Adiatmika, I. P. G., Wulanyani, N. M. S., & Sundari, L. P. R. (2024). Sidhakarya state conditioning in improving shooting accuracy of petanque athletes in Bali. Retos, 59, 1071–1080. https://doi.org/10.47197/retos.v59.105523
Strehli, I., Burns, R. D., Bai, Y., Ziegenfuss, D. H., Block, M. E., & Brusseau, T. A. (2021). Mind–body physical activity interventions and stress-related physiological markers in educational settings: A systematic review and meta-analysis. In International Journal of Environmental Research and Public Health (Vol. 18, Issue 1, pp. 1–24). MDPI AG. https://doi.org/10.3390/ijerph18010224
Wu, J., Yang, C., Yang, M., Liang, Z., Wu, Y., Kong, X., Fan, H., Wang, S., Ning, C., Xiao, W., Jin, Y., Yi, J., & Yuan, Z. (2022). The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin. Ecotoxicology and environmental safety, 237, 113531. https://doi.org/10.1016/j.ecoenv.2022.113531
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