The effectiveness of Muay Thai training on stress biomarkers and body composition in young adults
DOI:
https://doi.org/10.47197/retos.v72.116896Keywords:
Muay Thai, Stress, Salivary biomarkers, Body composition, Sympathetic nervous systemAbstract
Introduction: Rising stress levels and poor body composition among university students necessitate effective physical interventions. Muay Thai, a high-intensity martial art combining aerobic and anaerobic demands, may offer dual benefits for metabolic health and stress reduction, though empirical evidence remains limited.
Objective: This study examined the effects of an 8-week Muay Thai training (MTT) program on stress biomarkers (sAA) muscle mass and body fat percentage compared to brisk walking (BWE) in university students.
Methodology: Sixty-four students were randomized into MTT (n=32) or BWE (n=32) groups. Both interventions consisted of 60-minute sessions, 3 time/week for 8-weeks, with intensity maintained at 60–80% of maximum heart rate. Body composition (BMI, body fat %, visceral fat) and sAA levels were assessed at baseline, 6-weeks, and post-intervention. Statistical analyses included paired t-tests and between-group comparisons (p<0.05).
Results: The MTT group showed superior reductions in body fat % (31.59% to 30.06%, p=0.029) and visceral fat (7.63 to 6.94, p=0.016) versus BWE. Energy expenditure was significantly higher in MTT (532 Kcal/session vs. 494 Kcal, p=0.011). While sAA levels transiently increased at 6 weeks in both groups (adaptation phase), MTT demonstrated a greater decline by 8 weeks (51.25 to 50.13 KU/L vs. BWE: 51.71 to 53.47 KU/L, p=0.041), indicating sustained stress modulation.
Conclusions: Muay Thai training significantly improves body composition and reduces sympathetic nervous system activity compared to moderate aerobic exercise. These findings support its integration into university health programs as a time-efficient, dual-purpose intervention. Future research should investigate long-term adherence and psycho-social mechanisms underlying its stress-reducing effects.
References
Aguiar, R. S., Lopes, G. C., Pinto de Castro, J. B., Prince, V. A., Mazini Filho, M. L., Nogueira da Gama, D. R., … Vale, R. G. (2021). Effects of high-intense resistance training on salivary cortisol in trained individuals: a systematic review (Efectos del entrenamiento de resistencia de alta intensidad sobre el cortisol salival en individuos entrenados: una revisión sistemática). Retos, 41, 265–271. https://doi.org/10.47197/retos.v0i41.82770
Azarbayjani, M. A., Fatolahi, H., Rasaee, M. J., Peeri, M., & Babaei, R. (2011). The effect of exercise mode and intensity of sub-maximal physical activities on salivary testosterone to cortisol ratio and α-amylase in young active males. International journal of exercise science, 4(4), 283- 293. https://doi: 10.70252/TLCE3265
Binder, E.B. & Nemeroff, C.B. (2010). The CRF system, stress, depression and anxiety insights from human genetic studies. Molecular psychiatry, 15(6), 574-588. https://doi:https://doi.org/ 10.1038/mp.2009.141.
Cannataro, R., Cione, E., Gallelli, L., Marzullo, N., & Bonilla, D. A. (2020). Acute effects of supervised making weight on health markers, hormones and body composition in muay thai fighters. Sports,8(10), 37. https://doi.org/10.3390/sports8100137
Carlson, L. E., Speca, M., Patel, K. D., & Goodey, E. (2004). Mindfulness-based stress reduction in relation to quality of life, mood, symptoms of stress and levels of cortisol, dehydroepiandrosterone sulfate (DHEAS) and melatonin in breast and prostate cancer outpatients. Psychoneuroendocrinology, 29(4), 448-474. https://doi.org/10.1016/S0306-4530(03)00054-4
Chipchase, L., Davidson, M., Blackstock, F., Bye, R., Clothier, P., Klupp, N., ... & Williams, M. (2017). Conceptualising and measuring student disengagement in higher education: A synthesis of the literature. International Journal of Higher Education, 6(2), 31-42. https://doi.org/10.5430/ijhe.v6n2p31
Cohen, S., Janicki-Deverts, D. & Miller, G.E. (2007). Psychological stress and disease. The Journal of the American Medical Association JAMA, 298(14),1685-1687. https://doi.org/10.1001/jama.298.14.1685.
Demetriou, P. (2024). Supporting the Mental Health and Wellbeing of Learners in Post-16 Education. Taylor & Francis.
Du, C., Zan, M. C. H., Cho, M. J., Fenton, J. I., Hsiao, P. Y., Hsiao, R., ... & Tucker, R. M. (2021). The effects of sleep quality and resilience on perceived stress, dietary behaviors, and alcohol misuse: a mediation-moderation analysis of higher education students from Asia, Europe, and North America during the COVID-19 pandemic. Nutrients, 13(2), 442. https://doi.org/10.3390/nu13020442
Engert, V., Vogel, S., Efanov, S. I., Duchesne, A., Corbo, V., Ali, N., & Pruessner, J. C. (2011). Investigation into the cross-correlation of salivary cortisol and alpha-amylase responses to psychological stress. Psychoneuroendocrinology, 36(9), 1294-1302. https://doi.org/10.1016/j.psyneuen.2011.02.018
Fatma, H., Srivastava, P., Johri, P., Rajput, M. S., Syed, S., & Trivedi, M. (2024). Hypertension and Cardiovascular Problems-An Outlook Associated with Adolescents' Lifestyles. In Lifestyle Diseases in Adolescents: Diseases, Disorders, and Preventive Measures. Bentham Science Publishers, 88-107. https://doi.org/10.2174/97898152744311240101
Fuentes-Barría, H., Sperberg-Aniñir, A., Peña-Gallegos, M. F., Aguilera-Eguía, R., & Alarcón-Rivera, M. (2024). Effects of physical activity on salivary pH and cariogenic risk in Chilean musicians from the Biobío region. Cross-sectional study. Retos, 61, 583–588. https://doi.org/10.47197/retos.v61.109671
Garnefski, N., Legerstee, J., Kraaij, V., van Den Kommer, T., & Teerds, J. A. N. (2002). Cognitive coping strategies and symptoms of depression and anxiety: A comparison between adolescents and adults. Journal of adolescence, 25(6), 603-611. https://doi.org/10.1006/jado.2002.0507
Gerber, M., Brand, S., Herrmann, C., Colledge, F., Holsboer-Trachsler, E., & Pühse, U. (2014). Increased objectively assessed vigorous-intensity exercise is associated with reduced stress, increased mental health and good objective and subjective sleep-in young adults. Physiology & behavior, 135, 17-24. https://doi.org/10.1016/j.physbeh.2014.05.047
Granger, D.A., Johnson, S.B., Szanton, S.L. & Out, D. (2012). Incorporating Salivary Biomarkers into Nursing Research: An Overview and Review of Best Practices. Biological Research for Nursing, 14(4), 347–356. https://doi.org/ 10.1177/1099800412443892.
Hackett, R.A. & Steptoe, A. (2017). Type 2 diabetes mellitus and psychological stress - a modifiable risk factor. Nature Reviews Endocrinology, 13(9), 547-560. https://doi.org/ 10.1038/nrendo.2017.64.
Johnson, K. F. (2022). Symptoms of anxiety in college students and the influence of social determinants of health. Journal of College Student Psychotherapy, 36(4), 339-354. https://doi.org/10.1080/87568225.2020.1827114
Kang, Y. (2010). Psychological stress-induced changes in salivary alpha amylase and adrenergic activity. Nursing & Health Sciences, 12, 477-84. https://doi.org/10.1111/j.1442-2018.2010.00562.x.
Kono, Y., Kubota, A., Taira, M., Katsuyama, N. & Sugimoto, K. (2018). Effects of oral stimulation with capsaicin on salivary secretion and neural activities in the autonomic system and the brain. Journal of Dental Sciences, 13(2)13,116-23. https://doi.org/10.1016/j.jds.2017.08.007.
Lederer, A. M., & Hoban, M. T. (2022). The development of the American College Health Association-National College Health Assessment III: An improved tool to assess and enhance the health and well-being of college students. Journal of American College Health, 70(6), 1606-1610. https://doi.org/10.1080/07448481.2020.1834401
Lehmann, S., Gärtner Askeland, K., & Hysing, M. (2021). Sleep among youths in foster care: Associations with potentially traumatic events, PTSD and mental health. Child & Family Social Work, 26(1), 111-121. https://doi.org/10.1111/cfs.12794
Mahyudi, Y. V., Ningrum, D. T. M., Hafizah, H., & Setyawan, D. A. (2025). Effect of Structured Physical Activity Programs on Physical Fitness and Body Compotition Among Junior High School: A Randomized Controlled Trial: English. Retos, 68, 249–257. https://doi.org/10.47197/retos.v68.111620
Marcus, C., Danielsson, P., & Hagman, E. (2022). Pediatric obesity—Long‐term consequences and effect of weight loss. Journal of internal medicine, 292(6), 870-891. https://doi.org/10.1111/joim.13547
Nagai, A., Kubota, M. & Higashiyama, Y. (2020). Relationship between taste acuity and fatigue or stress in Japanese elementary school children. Journal of Japan Society of Nutrition and Food Sciences, 66, 249-54. https://doi:10.4327/jsnfs.66.249.
Nater, U.M., Rohleder, N., Schlotz, W., Ehlert, U. & Kirschbaum, C. (2007). Determinants of the diurnal course of salivary alpha-amylase. Psychoneuroendocrinology, 32, 392-401. https://doi.org/10.1016/j.psyneuen.2007.02.007.
Park, J., & Han, S.H. (2003). The effect of exercise program on health and depression in the elderly. Journal of Korean Community Nursing, 33(2), 220-227. https://doi.org/10.4040/jkan.2003.33.2.220.
Pennebaker, J. W., Colder, M., & Sharp, L. K. (1990). Accelerating the coping process. Journal of personality and social psychology, 58(3), 528-537. https://doi.org/10.1037/0022-3514.58.3.528
Phanpheng, Y., & Laha, W. (2023). The Efficiency of Thai Rhythm Calisthenics Movement on Salivary Alpha-Amylase and Health Performance in the Elderly. International Journal of Kinesiology and Sports Science, 11(2), 25-34. https://doi.org/10.7575/aiac.ijkss.v.11n.2p.25
Pineda-Espejel, H. A., Trejo, M., García, K. B., Garza, K. J., Vázquez-Jiménez, G., Machado-Contreras, J. R., … Rodríguez, S. (2020). Respuesta de cortisol salival y ansiedad precompetitiva en nadadores (Salivary cortisol response and precompetitive anxiety in swimmers). Retos, 38, 1–7. https://doi.org/10.47197/retos.v38i38.72229
Pranoto, A., Rejeki, P. S., Miftahussurur, M., Yosika, G. F., Ihsan, M., Herawati, L., … Halim, S. (2024). Aerobic Exercise Increases Release of Growth Hormone in the Blood Circulation in Obese Women. Retos, 51, 726–731. https://doi.org/10.47197/retos.v51.99944
Prince, E. J., Siegel, D. J., Carroll, C. P., Sher, K. J., & Bienvenu, O. J. (2021). A longitudinal study of personality traits, anxiety, and depressive disorders in young adults. Anxiety, Stress, & Coping, 34(3), 299-307. https://doi.org/10.1080/10615806.2020.1845431
Rapkiewicz, J. A., Nunes, J. P., Mayhew, J. L., Ribeiro, A. S., Nabuco, H. C., Fávero, M. T., & Amarante Do Nascimento, M. (2018). Effects of Muay Thai training frequency on body composition and physical fitness in healthy untrained women. Journal Sports Med Phys Fitness, 58(12), 1808-1814.
Simón Mora, R. M., Sánchez Oliver, A. J., Suárez Carmona, W., & González Jurado, J. A. (2021). Effect of a physical exercise program on physical fitness and visceral fat in people with obesity. Retos, 39, 723–730. https://doi.org/10.47197/retos.v0i39.78997
Steptoe, A., Dockray, S., Wardle, J. (2009). Positive affect and Psychobiological processes relevant to health. Journal Personal, 77, 1747–1776. https://doi.org/10.1111/j.1467-6494.2009.00599.x
World Health Organization. (2023). Noncommunicable diseases and mental health in small island developing states. World Health Organization.
Yamaguchi, M., Kanemaru, M., Kanemori, T. & Mizuno, Y. (2003). Flow-injection-type biosensor system for salivary amylase activity. Biosensors & Bioelectronics, 18, 835-840. https://doi.org/ 10.1016/s0956-5663(03)00007-1.
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